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Ken-A-Vision Experiment 3

GOING THROUGH A PHASE:
Mitosis: cellular reproduction

Objectives: The objective of this activity is to further understand
the processes involved in cellular reproduction.

Suggested materials:
· Microprojector
· Onion
· Prepared slides


Vocabulary:
· Mitosis
· Interphase
· Prophase
· Metaphase

· Anaphase
· Telophase
· Daughter cells

NOTES TO THE TEACHER:

1. A thin piece of onion skin can be used to observe organelles in plant cells. Carefully peel a thin strip of onion and place on a slide, stain with iodine. One or two drops are sufficient. (Iodine can be purchased in any
drug store.)

2.
Place the slide on the microprojector for viewing and search for organelles such as nucleus of the cell.

3.
Prepared slides can be used to study the changes which occur during mitosis: compare the chromatin of the surrounding cells with the chromosomes of the dividing cells using a prepared slide of an onion root tip.

4.
Also observe the prepared slides and discuss the phase of mitosis which some of the cells are undergoing. Compare and contrast the cellular structures in the various phases of mitosis.

ENRICHMENT:

1. Have students draw and label the various stages of mitosis.

2.
Divide the students into lab groups. Suggest three-dimensional diagrams to students using edible or non-edible materials showing mitosis. Each group can then show and discuss their diagram with the class.

3.
Investigate the role of DNA in living cells through library resources.

4.
Have students draw and label stages of mitosis on index cards. When the cards are flipped, the cells appear to go through mitosis.

Activity: GOING THROUGH A PHASE!
Mitosis: cellular reproduction

Purpose: The purpose of this activity is to observe the various stages of mitosis on prepared slides as well as slides prepared by the student.

Procedure:
1. A thin piece of onion skin can be used to observe organelles in plant cells. Carefully peel a thin strip of onion and place on a slide, stain with iodine. One or two drops are sufficient. (Iodine can be purchased in any drug store.)

2.
Place the slide on the microprojector for viewing and search for organelles such as nucleus of the cell.

3.
Observe the commercially prepared slides (available from Ken-A-Vision) showing the various stages of mitosis.
a. What organelles stand out in the stain?
b. Draw each stage of mitosis from the slides and label the structures of the cell.
c. What changes take place in the chromatin as the cell divides? d. Where is the DNA found in the cell?
e. Carefully study the cells.
f. Locate cells undergoing mitosis by looking for partially divided pairs of cells. How do these dividing cells compare with those not undergoing mitosis?
g. Trace several examples of various phases of cell division of the dividing cells.

Conclusion:

1. Each lab group should show the slides which they prepared to the class using the Microprojector.

2.
What factors cause a cell to divide?

3.
How do you know cells are dividing?

4.
Are the daughter cells exactly alike after mitosis?

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